Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices

• Published in: Analyst (London) Vol. 138; no. 3; pp. 845 - 849
• Main Authors: Lafleur, Josiane P, Kwapiszewski, Radoslaw, Jensen, Thomas G, Kutter, Jörg P
• Format: Journal Article
• Language: English
• Published: England 01.01.2013
• Subjects: Alkynes - chemistry; Biochips; Biomolecules; Biotin - chemistry; Density; Devices; Fluorescent Dyes - chemistry; Microfluidic Analytical Techniques; Microfluidics; Patterning; Photochemical; Polymers - chemistry; Spectrometry, Fluorescence - instrumentation; Streptavidin - chemistry; Sulfhydryl Compounds - chemistry; Surface Properties; Thiols
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c2an36424g

The suitable optical properties of thiol-ene polymers combined with the ease of modifying their surface for the attachment of recognition molecules make them ideal candidates in many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips. This work focuses on thiol-ene substrates featuring an excess of thiol groups at their surface. The thiol-ene stoichiometric composition can be varied to precisely control the number of surface thiol groups available for surface modification up to an average surface density of 136 ± 17 SH nm −2 . Biotin alkyne was patterned directly inside thiol-ene microchannels prior to conjugation with fluorescently labelled streptavidin. The surface bound conjugates were detected by evanescent wave-induced fluorescence (EWIF), demonstrating the success of the grafting procedure and its potential for biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in microfluidic thiol-ene chips.