Induction of somatic embryogenesis in Iris hollandica Hort. cv. 'Bronze Queen'

• Published in: The journal of horticultural science & biotechnology Vol. 80; no. 1; pp. 135 - 138
• Main Authors: Fidalgo, F, Santos, A, Oliveira, N, Santos, I, Salema, R
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 2005
• Subjects: callus; cell differentiation; culture media; developmental stages; histology; in vitro culture; in vitro regeneration; Iris hollandica; methodology; micropropagation; ornamental plants; somatic embryogenesis
• ISSN: 1462-0316; 2380-4084
• DOI: 10.1080/14620316.2005.11511904

Twin-scale explants of Iris hollandica cv. 'Bronze Queen' produced callus when cultured on a modified Murashige and Skoog medium supplemented with 9.0, 18.1, 27.2 or 36.2 µM 2,4-dichlorophenoxyacetic acid (2,4-D). Induction of embryogenic callus was obtained at 9.0 or 18.1 µM 2,4-D, while higher concentrations of the auxin (27.2 or 36.2 µM) were not effective. Embryo-like structures developed on the surface of embryogenic calli. Somatic embryo maturation was achieved when the bipolar structures produced were cultured on medium without auxin. To improve embryo germination, the developing embryos were transferred to medium supplemented with 0.95 µM abscisic acid (ABA). In this medium, mature embryos developed into normal plantlets. Histological studies confirmed that the structures exhibiting bipolar differentiation were somatic embryos. Regeneration of I. hollandica cv. 'Bronze Queen' plants through somatic embryogenesis was thus established.