Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents

While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we re...

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Published inCryobiology Vol. 86; pp. 130 - 133
Main Authors Powell-Palm, Matthew J., Zhang, Yanfang, Aruda, Justin, Rubinsky, Boris
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.02.2019
Subjects
Animals
Cell Survival - drug effects
Cold Temperature
Cryopreservation - methods
Cryoprotective agents
Cryoprotective Agents - analysis
Freezing
Islets of Langerhans - cytology
Isochoric cryopreservation
Models, Animal
Organ cryopreservation
Osmosis
Pancreatic islets
Rats
Rats, Sprague-Dawley
Thermodynamics
Organ cryopreservation
Pancreatic islets
Cryoprotective agents
Isochoric cryopreservation
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Abstract While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value. •Isochoric (constant) volume system without cryoprotectants.•Pancreatic islets preservation for up to three days.•Pancreatic islets preservation at - 3 °C.
AbstractList While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value.
While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value. •Isochoric (constant) volume system without cryoprotectants.•Pancreatic islets preservation for up to three days.•Pancreatic islets preservation at - 3 °C.
Author Zhang, Yanfang
Aruda, Justin
Powell-Palm, Matthew J.
Rubinsky, Boris
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10.1097/MPA.0b013e3181dab19a
10.1016/j.bbrc.2018.01.140
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Keywords Organ cryopreservation
Pancreatic islets
Cryoprotective agents
Isochoric cryopreservation
Language English
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Snippet While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant...
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SubjectTerms Animals
Cell Survival - drug effects
Cold Temperature
Cryopreservation - methods
Cryoprotective agents
Cryoprotective Agents - analysis
Freezing
Islets of Langerhans - cytology
Isochoric cryopreservation
Models, Animal
Organ cryopreservation
Osmosis
Pancreatic islets
Rats
Rats, Sprague-Dawley
Thermodynamics
Title Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents
URI https://dx.doi.org/10.1016/j.cryobiol.2019.01.003
https://www.ncbi.nlm.nih.gov/pubmed/30629949
https://search.proquest.com/docview/2179348812
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