Isochoric conditions enable high subfreezing temperature pancreatic islet preservation without osmotic cryoprotective agents

• Published in: Cryobiology Vol. 86; pp. 130 - 133
• Main Authors: Powell-Palm, Matthew J., Zhang, Yanfang, Aruda, Justin, Rubinsky, Boris
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.02.2019
• Subjects: Animals; Cell Survival - drug effects; Cold Temperature; Cryopreservation - methods; Cryoprotective agents; Cryoprotective Agents - analysis; Freezing; Islets of Langerhans - cytology; Isochoric cryopreservation; Models, Animal; Organ cryopreservation; Osmosis; Pancreatic islets; Rats; Rats, Sprague-Dawley; Thermodynamics; Organ cryopreservation; Pancreatic islets; Cryoprotective agents; Isochoric cryopreservation
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2019.01.003

While biological systems are typically studied under isobaric (constant pressure) conditions, recent reports on the bio-thermodynamics of isochoric (constant volume) systems point to their potential for subfreezing-temperature preservation of biological matter. This preliminary study, in which we report that pancreatic islets can survive multi-day preservation at high subfreezing temperatures in an isochoric chamber without osmotic cryoprotective agents (CPA), highlights the potential of isochoric cryopreservation in an application of clinical value. •Isochoric (constant) volume system without cryoprotectants.•Pancreatic islets preservation for up to three days.•Pancreatic islets preservation at - 3 °C.