Gryllotalpicola soli sp. nov., isolated from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 64; no. Pt 12; pp. 4079 - 4083
• Main Authors: Moon, Ji-Young, Kim, Soo-Jin, Hamada, Moriyuki, Ahn, Jae-Hyung, Weon, Hang-Yeon, Suzuki, Ken-Ichiro, Yoon, Jung-Hoon, Kwon, Soon-Wo
• Format: Journal Article
• Language: English
• Published: England 01.12.2014
• Subjects: Actinomycetales - classification; Actinomycetales - genetics; Actinomycetales - isolation & purification; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phylogeny; Republic of Korea; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2 - chemistry
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.067710-0

A novel Gram-stain-positive, short rod-shaped, non-flagellated and mesophilic strain, KIS12-7(T), isolated from a soil sample collected from Daecheong-Island in Ongjin County, Republic of Korea, was studied using a polyphasic approach. Phylogenetic trees based on the 16S rRNA gene sequence revealed that the novel strain was a member of the genus Gryllotalpicola, showing more than 97.0 % sequence similarity with Gryllotalpicola daejeonensis RU-04(T) (98.0 %), Gryllotalpicola koreensis RU-16(T) (97.7 %) and Gryllotalpicola kribbensis PU-02(T) (97.3 %). However, DNA-DNA relatedness values demonstrated that strain KIS12-7(T) could be clearly distinguished from closely related species of the genus Gryllotalpicola. The cell-wall peptidoglycan of strain KIS12-7(T) was of the type B2 and the acyl type was acetyl. The predominant menaquinones were MK-11 and MK-10. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unknown phosphoglycolipid, one unknown glycolipid, one unknown phospholipid and one unknown lipid. The G+C content of the genomic DNA was 72.1 mol%. On the basis of the evidence presented, strain KIS12-7(T) is a representative of a novel species of the genus Gryllotalpicola, and the name Gryllotalpicola soli sp. nov. is proposed; the type strain is KIS12-7(T) ( = DSM 27182(T) = KACC 17302(T) = NBRC 109659(T)).