Antimicrobial properties of actinomycetes from the soil of Himalaya
Twelve actinomycetes strains were isolated from the soil samples of the Himalaya and screened for their antimicrobial activity. Their cultural characteristics in different culture media were studied. ERIH-44 showed both antibacterial and antifungal activity. The 16S rRNA pattern of ERIH-44 strongly...
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Published in | Journal de mycologie médicale Vol. 20; no. 1; pp. 15 - 20 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English French |
Published |
Paris
Elsevier SAS
01.03.2010
Masson |
Subjects | |
Online Access | Get full text |
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Summary: | Twelve actinomycetes strains were isolated from the soil samples of the Himalaya and screened for their antimicrobial activity. Their cultural characteristics in different culture media were studied. ERIH-44 showed both antibacterial and antifungal activity. The 16S rRNA pattern of ERIH-44 strongly suggested that it belongs to the genus
Bacillus. The morphological and physiological characters were also recorded. ERIH-44 had the ability to produce amylase. The antimicrobial activity was assessed using broth microdilution technique. The ethyl acetate extract of ERIH-44 was tested against bacteria and fungi, and showed the following MIC values:
Bacillus subtilis (<
15.62
μg/ml),
Staphylococcus aureus (<
15.62
μg/ml),
Escherichia coli (125
μg/ml) and
Pseudomonas aeruginosa (500
μg/ml),
Botrytis cinerea (500
μg/ml) and
Trichophyton mentagrophytes (1000
μg/ml).
Douze souches d’actinomycètes ont été isolées dans des échantillons de sol de l’Himalaya et testées pour leur activité antimicrobienne. Leurs caractéristiques de culture ont été étudiées sur différents milieux. La souche ERIH-44 a montré une activité antibactérienne et antifongique. Le profil de l’ARNr 16S d’ERIH-44 a fortement suggéré qu’il appartient au genre
Bacillus. Les caractères morphologiques et physiologiques ont été également étudiés. La souche ERIH-44 a la capacité de produire de l’amylase. L’activité antimicrobienne a été évaluée en utilisant la technique de microdilution en bouillon. L’extrait à l’acétate d’éthylique d’ERIH-44 a été testé contre des bactéries et des champignons qui ont montré les valeurs de concentration minimale inhibitrice suivantes :
Bacillus subtilis (<
15,62
μg/ml),
Staphylococcus aureus (<
15,62
μg/ml),
Escherichia coli (125
μg/ml),
Pseudomonas aeruginosa (500
μg/ml),
Botrytis cinerea (500
μg/ml) et
Trichophyton mentagrophytes (1000
μg/ml). |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1156-5233 1773-0449 |
DOI: | 10.1016/j.mycmed.2009.11.002 |