Study on effects of preparation method on the structure and antioxidant activity of protein-Tremella fuciformis polysaccharide complexes by asymmetrical flow field-flow fractionation

• Published in: Food chemistry Vol. 384; p. 132619
• Main Authors: Chen, Xue, Tian, Yiling, Zhang, Jie, Li, Yueqiu, Zhang, Wenhui, Zhang, Jing, Dou, Yuwei, Dou, Haiyang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2022
• Subjects: Antioxidant activity; Antioxidants; Asymmetrical flow field-flow fractionation; Basidiomycota; Dietary Carbohydrates; Fractionation, Field Flow - methods; Humans; Low density lipoprotein; Polysaccharide; Polysaccharides; Asymmetrical flow field-flow fractionation; Low density lipoprotein; Polysaccharide; Antioxidant activity
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2022.132619

•Low density lipoprotein-Tremella fuciformis polysaccharide complexes were prepared.•Effect of the preparation method on the structure of the complex was investigated.•Field-flow fractionation was employed for the characterization of the complex.•The antioxidant activity of the complex was studied. Oxidation is an essential biological process for human life. In this study, low density lipoprotein-Tremella fuciformis polysaccharide (LDL-TFP) complexes were prepared by electrostatic and covalent methods. The effects of preparation method on the structure and antioxidant activity of LDL-TFP complexes were investigated by asymmetrical flow field-flow fractionation (AF4) coupled with ultraviolet–visible (UV/Vis), multiangle light scattering (MALS), and differential refractive index (dRI) detectors. The results showed that the electrostatic LDL-TFP complexes had a spherical structure, while the covalent LDL-TFP complexes had a rod-like structure as indicated by the ratio of Rg (radius of gyration) to Rh (hydrodynamic radius). Moreover, the results revealed that the antioxidant activity of the LDL-TFP complexes on the HepG2 could be related to the structure of LDL-TFP complexes. The antioxidant activity of LDL-TFP complexes formed by LDL modified with phospholipase A2 was further enhanced. This study would help expand the application of TFP.