Integrative transcriptomics and proteomics analysis constructs a new molecular model for ovule abortion in the female-sterile line of Pinus tabuliformis Carr

• Published in: Plant science (Limerick) Vol. 294; p. 110462
• Main Authors: Zhang, Min, Li, Wenhai, Feng, Jun, Gong, Zaixin, Yao, Yang, Zheng, Caixia
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.05.2020
• Subjects: Female-sterile mutant; Free nuclear mitosis; Integrative analysis; Megagametophyte; Ovule abortion; Pinus tabuliformis Carr; dUTP; RPKM; RNA-seq; KEGG; CCs3; RFC3; PARP2; SMC; CMT; SLe; ss-DNA; PRI1; RNase H2B; Free nuclear mitosis; SLl; RF; ds-DNA; DNA MTase; cor-DEGs; RR; DNA-PKcs; FG; dTTP; PCNA; CytC; FL; dNTP; FNMM; ETF-QO; Female-sterile mutant; MET1; FDR; POLD1; SL; FLe; DSBs; CCs1; RRM1; Megagametophyte; FLl; CDKA; GO; RPA1; RPA2; Pinus tabuliformis Carr; CDC2; Integrative analysis; POLA1; Ovule abortion; CHROMO; MCM; POLE2; iTRAQ; DEPs; DETs; DUTPase
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2020.110462

•In SL ovules, low expressions of MCM complex, etc. led to abnormal DNA replication.•The expressions of PARP2, etc. implied the accumulation of DNA DSBs.•SL ovules failed to repair the DNA damage, and the PCD occurred.•The deficiency of ETF-QO hindered the progress of FNMM.•FNMM stopped, which resulted in the ovule abortion of SL. Ovule development is critical to plant reproduction and free nuclear mitosis of megagametophyte (FNMM) is vital for ovule development. However, most results of ovule development were based on the studies in angiosperms, and its molecular regulation remained largely unknown in gymnosperms, particularly, during FNMM. In this context, we studied the genome-wide difference between sterile line (SL) and fertile line (FL) ovules using transcriptomics and proteomics approaches in Pinus tabuliformis Carr. Comparative analyses revealed that genes involved in DNA replication, DNA damage repair, Cell cycle, Apoptosis and Energy metabolism were highlighted. Further results showed the low expressions of MCM 2-7, RRM1, etc. perhaps led to abnormal DNA replication and damage repair, and the significantly different expressions of PARP2, CCs1, CCs3, etc. implied that the accumulated DNA double-stranded breaks were failed to be repaired and the cell cycle was arrested at G2/M in SL ovules, potentially resulting in the occurrence of apoptosis. Moreover, the deficiency of ETF-QO might hinder FNMM. Consequently, FNMM stopped and ovule aborted in SL ovules. Our results suggested a selective regulatory mechanism led to FNMM half-stop and ovule abortion in P. tabuliformis and these insights could be exploited to investigate the molecular regulations of ovule development in woody gymnosperms.