Differential effects of a partially purified preparation of slow-reacting substance of anaphylaxis on guinea pig tracheal spirals and parenchymal strips

• Published in: The Journal of clinical investigation Vol. 63; no. 1; pp. 1 - 5
• Main Authors: Drazen, J M, Lewis, R A, Wasserman, S I, Orange, R P, Austen, K F
• Format: Journal Article
• Language: English
• Published: United States 01.01.1979
• Subjects: Airway Obstruction - chemically induced; Animals; Guinea Pigs; Histamine - pharmacology; In Vitro Techniques; Lung - drug effects; Lung - physiology; Male; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Organ Specificity; SRS-A - pharmacology; Trachea - drug effects; Trachea - physiology
• ISSN: 0021-9738
• DOI: 10.1172/JCI109262

The contractile effects of partially purified slow-reacting substance of anaphylaxis (SRS-A) and histamine were compared on isolated guinea pig tracheal spirals and parenchymal strips. Histamine was equally active on both isolated tissues in a concentration-related fashion. SRS-A (0.1--10.0 U/ml) produced a concentration-related effect on parenchymal strips, whereas the tracheal spiral was 100 times less sensitive to this mediator. The contractile activity of SRS-A on parenchymal strips was diminished by incubation with limpet arylsulfatase and antagonized by FPL 55712, a known SRS-A antagonist. SRS-A, further purified by high pressure liquid chromatography, also demonstrated this preferential activity on guinea pig parenchymal strips. These data are consistent with the hypothesis, based on previous in vivo observations, that SRS-A is a selective peripheral airway constrictor.