Establishment of a rescue system for an autonomous Parvovirus mink enteritis virus

•A full-length infectious clone (pMEV) of mink enteritis virus was constructed.•pMEV was infectious when transfected into F81 cells.•rMEV retained the biological properties of MEV-L. Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Fel...

Full description

Saved in:
Bibliographic Details
Published inVirus research Vol. 183; pp. 1 - 5
Main Authors Yuan, Daoli, Wang, Jigui, Li, Zhili, Mao, Yaping, Sun, Jia-zeng, Xi, Ji, Wang, Shuang, Hou, Qiang, Yi, Bao, Liu, Weiquan
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 21.04.2014
Subjects
Animals
Biological properties
Cats
Cell Line
Genetic marker
Infectious clone
Mink enteritis virus
Mink enteritis virus - genetics
Mink enteritis virus - growth & development
Rescue virus
Reverse genetics
Reverse Genetics - methods
Virology - methods
Infectious clone
Rescue virus
Biological properties
Mink enteritis virus
Genetic marker
Reverse genetics
Online AccessGet full text

Cover

More Information
Summary:•A full-length infectious clone (pMEV) of mink enteritis virus was constructed.•pMEV was infectious when transfected into F81 cells.•rMEV retained the biological properties of MEV-L. Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Feline kidney cells (F81) were transfected with pMEV containing an engineered BamHI site that served as a genetic marker. The rescued virus was indistinguishable from its parental virus. The availability of a MEV infectious clone will facilitate studies of viral replication and pathogenicity and will permit the elucidation of determinants of the host range of the parvovirus.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2014.01.012