Evaluation of porous bacterial cellulose produced from foam templating with different additives and its application in 3D cell culture

Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simp...

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Published inInternational journal of biological macromolecules Vol. 234; p. 123680
Main Authors Lin, Shin-Ping, Singajaya, Stephanie, Lo, Tsui-Yun, Santoso, Shella Permatasari, Hsu, Hsien-Yi, Cheng, Kuan-Chen
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.04.2023
Subjects
3D cell culture
Animals
Bacterial cellulose
Biocompatible Materials - chemistry
Cell Adhesion
Cell Culture Techniques, Three Dimensional
Cellulose - chemistry
Foam templating
Mice
Porosity
Tissue Engineering - methods
Tissue scaffold
Tissue Scaffolds - chemistry
Foam templating
Tissue scaffold
Bacterial cellulose
3D cell culture
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Summary:Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as mechanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % ~ 93.67 %) compared to BC samples (44.52 % ~ 67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering. [Display omitted] •FBC/additive exhibited great swelling and reswelling ability.•FBC/additive presented great cell adhesion and proliferation.•NIH-3T3 cells can be cultured into deeper layers of FBC/additive.•Detailed characterizations of material property in FBC/additive.
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ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.123680