Description and quantification of micronucleus and nuclear abnormalities in erythrocytes of the sentinel green turtle (Chelonia mydas) with fluorescence microscopy

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis Vol. 887; p. 503596
• Main Authors: Guevara-Meléndez, Aida Mercedes, Comas-Garcia, Mauricio, Labrada-Martagón, Vanessa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2023
• Subjects: Animals; Biomarkers; Cheloniidae; Ecosystem; Environmental Pollutants; Erythrocytes; Genotoxicity; Green turtle; Humans; Micronucleus; Microscopy; Microscopy, Fluorescence; Turtles - physiology; Water Pollutants, Chemical; Biomarkers; Microscopy; Cheloniidae; Green turtle; Genotoxicity; Micronucleus
• ISSN: 1383-5718; 1879-3592; 1879-3592
• DOI: 10.1016/j.mrgentox.2023.503596

Contamination in marine ecosystems is of the most critical threats to marine turtles. The identification of useful biomarkers to detect and monitor the physiological and clinical effects of pollutants on these populations will allow early detection of alterations (e.g., mutagenic damages) that could risk their viability or favor the development of diseases, thus threatening the biodiversity of these ecosystems and human population. This study is aimed at describing and quantifying nuclear anomalies in peripheral blood erythrocytes of green turtles (Chelonia mydas) from three distinct foraging areas in Mexico (Akumal, Xcalak, and Punta Herrero). We developed a novel morphological index that could be used as a biomarker to identify abnormal nuclei in peripheral blood erythrocytes. Here we describe for the first time in C. mydas, with a species-specific staining protocol, distinct nuclear abnormalities such as blebbed, lobed, notched, eight shape nuclei, and binucleated cells. These nuclear abnormalities were present in > 90 % of the subjects (n = 30). Moreover, 50 % of the organisms presented erythrocytes with micronuclei. The number of nuclear abnormalities did not correlate with size of the green turtles or differ between sites, or health status. We found a higher frequency of green turtles with nuclear abnormalities in the southern region (Punta Herrero and Xcalak) with the highest frequency of micronucleus and buds. The former could be associated to the constant exposure to chemical pollutants of oceanographic origin in the southern coast of Quintana Roo. Furthermore, the increasing anthropogenic pollution in Akumal could also explain the highest variability in the number of nuclear abnormalities presented in resident individuals. We propose that a long-term monitoring programs of green turtle populations in the Mexican Caribbean that include a micronucleus test could be a useful to determine possible mutagenic damage in these animals. •Nuclear abnormalities not described before in sea turtles were present in 90 % of subjects.•Micronucleated erythrocytes were present in half of the sampled Caribbean green turtles.•The frequency of nuclear abnormalities did not correlate with body size.•The micronucleus test is a biomarker of foraging habitat conditions for C. mydas.