Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

The reactions of exo-cellulase （cellobiohydrolase, CBH） and endo-cellulase （endoglucanase, EG） were investigated by analyzing the insoluble residues of microcrystalline cellulose （MCC） and filter paper cellulose （FPC） during enzymatic hydrolysis. Molecular parameters including molecular weight and i...

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Published inChinese journal of chemical engineering Vol. 19; no. 5; pp. 773 - 778
Main Author 张名佳 苏荣欣 齐崴 杜若愚 何志敏
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.10.2011
Subjects
cellobiohydrolase
Cellulose
Degree of polymerization
Digestion
endoglucanase
Hydrolysis
Light scattering
relative molecular mass distribution
Scission
SEC
SEC-MALLS-DRI
Slopes
Solubilization
内切纤维素酶
内切葡聚糖酶
反应模式
商场
尺寸排阻色谱
纤维素酶水解
结晶度
endoglucanase
SEC-MALLS-DRI
cellobiohydrolase
relative molecular mass distribution
cellulose
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Summary:The reactions of exo-cellulase （cellobiohydrolase, CBH） and endo-cellulase （endoglucanase, EG） were investigated by analyzing the insoluble residues of microcrystalline cellulose （MCC） and filter paper cellulose （FPC） during enzymatic hydrolysis. Molecular parameters including molecular weight and its distribution, degree of polymerization, and radii of gyration were measured by size exclusion chromatography coupled with multi-angle laser light scattering. No significant change in MCC chains was found during the whole reaction period, indicating that CBH digestion follows a layer-by-layer solubilization manner. This reaction mode might be the major reason for slow enzymatic hydrolysis of cellulose. On the other hand, the degree of polymerization of FPC chains decreases rapidly in the initial reaction, indicating that EG digestion follows a random scission manner, which may create new ends for CBH easily. The slopes of the conformation plots for MCC and FPC increase gradually, indicating stronger chain stiffness of cellulose during hvdrolvsis
Bibliography:ZHANG Mingjia, SU Rongxin, QI Wei, DU Ruoyu,HE Zhimin（State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China）
The reactions of exo-cellulase （cellobiohydrolase, CBH） and endo-cellulase （endoglucanase, EG） were investigated by analyzing the insoluble residues of microcrystalline cellulose （MCC） and filter paper cellulose （FPC） during enzymatic hydrolysis. Molecular parameters including molecular weight and its distribution, degree of polymerization, and radii of gyration were measured by size exclusion chromatography coupled with multi-angle laser light scattering. No significant change in MCC chains was found during the whole reaction period, indicating that CBH digestion follows a layer-by-layer solubilization manner. This reaction mode might be the major reason for slow enzymatic hydrolysis of cellulose. On the other hand, the degree of polymerization of FPC chains decreases rapidly in the initial reaction, indicating that EG digestion follows a random scission manner, which may create new ends for CBH easily. The slopes of the conformation plots for MCC and FPC increase gradually, indicating stronger chain stiffness of cellulose during hvdrolvsis
cellulose, cellobiohydrolase, endoglucanase, relative molecular mass distribution, SEC-MALLS-DRI
11-3270/TQ
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1004-9541
2210-321X
DOI:10.1016/S1004-9541(11)60055-4