Isolation, characterization and bioactivities of the polysaccharides from Dicliptera chinensis (L.) Juss

• Published in: International journal of biological macromolecules Vol. 101; pp. 603 - 611
• Main Authors: Xu, Yourui, Gao, Ya, Zhong, Mingli, Li, Jun, Cao, Houkang, Huang, Simao, Wei, Riming, Zhang, Kefeng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2017
• Subjects: Aging - drug effects; Animals; Anti-aging activity; Antioxidant activity; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Dicliptera chinensis (L.) Juss; Ethanol - chemistry; Female; Mice; Molecular Weight; Pinaceae - chemistry; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Water - chemistry; DPPH 2; Antioxidant activity; Anti-aging activity; FT-IR; SOD; T-AOC; LPO; MDA; Vc; GPX; TFA; CR; Dicliptera chinensis (L.) Juss; DCP; CAT; T-SOD; GPC; GC; D-gal; ABTS
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.03.112

•The polysaccharides (DCP-1 and DCP-2) were extracted and isolated from Dicliptera chinensis (L.) Juss.•The structural characters, Mw and monosaccharide compositions of DCP-2 were analyzed.•In vitro antioxidant activities of DCPs were determined, and DCP-2 had stronger antioxidant activity.•Anti-aging activity of DCP-2 was evaluated using an aging model-induced by D-galactose in mice. The polysaccharides of Dicliptera chinensis (L.) Juss. (DCP-1 and DCP-2) were extracted and isolated using the methods of water extract–ethanol precipitate and sephadex column chromatography and characterized by gel permeation chromatography (GPC), Fourier transform infrared spectrometry (FT-IR) and gas chromatography (GC), respectively. The antioxidant activity of DCPs was evaluated by scavenging activity of DPPH, hydroxyl, superoxide anion and ABTS radical. Moreover, the anti-aging activity of DCP-2 was investigated using an aging model-induced by D-galactose (D-gal) in mice. The results show that the weight average molecular weight (Mw) of DCP-2 was 2 273Da with a narrow polydispersity index of 1.01, and it was a heteropolysaccharide and consisted of glucose, galactose, arabinose, rhamnose and mannose with a molar ratio of 3.20:2.54:1.69:1.58:1.00. DCP-2 had stronger antioxidant activity against DPPH, hydroxyl, superoxide anion and ABTS radical, while DCP-1 had hardly any antioxidant activity and DCP had weaker antioxidant activity. Furthermore, DCP-2 can enhance antioxidant capacity and had anti-aging activity against D-gal induced aging mice. These results proposed that DCP-2 might be developed as a potential functional food with the activity of anti-aging.