Polyurethane and cellulose acetate micro-nanofibers containing rosemary essential oil, and decorated with silver nanoparticles for wound healing application

• Published in: International journal of biological macromolecules Vol. 226; pp. 690 - 705
• Main Authors: Rather, Anjum Hamid, Khan, Rumysa Saleem, Wani, Taha Umair, Rafiq, Muheeb, Jadhav, Arvind H., Srinivasappa, Puneethkumar M., Abdal-hay, Abdalla, Sultan, Phalisteen, Rather, Sami-ullah, Macossay, Javier, Sheikh, Faheem A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.01.2023
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial mechanism; Cell viability; Humans; Metal Nanoparticles - chemistry; Nanofibers - chemistry; Oils, Volatile - pharmacology; Polyurethanes; Rosmarinus; Silver - chemistry; Tissue engineering; Wound Healing; Tissue engineering; Cell viability; Antibacterial mechanism
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.12.048

In this study, polyurethane (PU) and cellulose acetate (CA) electrospun fibers encapsulating rosemary essential oil (REO) and adsorbed silver (Ag) nanoparticles (NPs) were fabricated. The biologically inspired materials were analyzed for physicochemical characteristics using scanning electron microscopy, X-ray diffractometer, Fourier transform infrared, thermal gravimetric analysis, X-ray photoelectron spectroscopy, water contact angle, and water uptake studies. Results confirmed the presence of CA and Ag NPs on the PU micro-nanofibers increased the hydrophilicity from 107.1 ± 0.36o to 26.35 ± 1.06o. The water absorption potential increased from 0.07 ± 0.04 for pristine PU fibers to 12.43 ± 0.49 % for fibers with 7 wt% of CA, REO, and Ag NPs. The diffractometer confirmed the 2θ of 38.01°, 44.13o, and 64.33o, corresponding to the diffraction planes of Ag on the fibers. The X-ray photoelectron spectroscopy confirmed microfibers interfacial chemical interaction and surface changes due to CA, REO, and Ag presence. The inhibition tests on Staphylococcus aureus and Escherichia coli indicated that composites are antibacterial in activity. Moreover, synergistic interactions of REO and Ag NPs resulted in superior antibacterial activity. The cell viability and attachment assay showed improved hydrophilicity of the fibers, which resulted in better attachment of cells to the micro-nanofibers, similar to the natural extracellular matrix in the human body.