Acidic polysaccharides from black ear and silver ear mushrooms modulated the release and transport of glucose from gelatinised sorghum starch during digestion

• Published in: Food chemistry Vol. 411; p. 135426
• Main Authors: Tu, Juncai, Adhikari, Benu, Brennan, Margaret Anne, Luo, Siyuan, Cheng, Ping, Bai, Weidong, Brennan, Charles Stephen
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.06.2023
• Subjects: Acidic mushroom polysaccharides; Agaricales; Caco-2 Cells; Digestion; Glucose; Glucose diffusion; Glucose transport; Humans; Polysaccharides - pharmacology; Sorghum; Sorghum starch; Starch; α-Glucosidase; Sorghum starch; Caco-2 cells; α-Glucosidase; Glucose diffusion; Glucose transport; Acidic mushroom polysaccharides
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2023.135426

[Display omitted] •In vitro digestion/Caco-2 cells model was used for glucose transport study.•Acidic mushroom polysaccharides reduced the amount of transported glucose.•Acidic mushroom polysaccharides inhibited α-glucosidase activity through mixed type.•Hypoglycaemic function of mushroom polysaccharides is related to their structure. This study investigated the ability of acidic polysaccharides from Auricularia auricula-judae (AAP) and Tremella fuciformis (TFP) mushrooms to modulate starch digestion and absorption. Gelatinised sorghum starch was used as starch-rich material, and its digestion and glucose transport were determined through in vitro digestion/Caco-2 cells model. Results showed that fortification with 0.6% AAP/TFP increased the proportion of high molecular weight α-dextrin and delayed glucose diffusion from digested starch gels. Gelatinisation of sorghum starch with AAP and TFP reduced the amount of transported glucose by 34.2% and 38.7%, respectively. This reduction was related to the inhibition of AAP/TFP on α-glucosidase and the difficulty in the hydrolysis of high molecular weight maltooligosaccharides. The potential bonding of AAP/TFP to glucose transporter (SGLT1) also impeded glucose transport. The findings suggest that AAP/TFP could act as natural hypoglycaemic agents used in starch-based foods and provide a better understanding of the hypoglycaemic mechanism of mushroom polysaccharides.