Polymorphism of legumin subunits from field bean (Vicia faba L. var. minor) and its relation to the corresponding multigene family
Legumin, which amounts to approximately 55% of the seed protein in field beans (Vicia faba L. var. minor), is a representative of the 12S storage globulin family. The 12S storage globulins are hexameric holoprotein molecules composed of different types of polymorphic subunits encoded by a multigene...
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Published in | Theoretical and applied genetics Vol. 86; no. 7; p. 867 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
01.08.1993
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Subjects | |
Online Access | Get more information |
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Summary: | Legumin, which amounts to approximately 55% of the seed protein in field beans (Vicia faba L. var. minor), is a representative of the 12S storage globulin family. The 12S storage globulins are hexameric holoprotein molecules composed of different types of polymorphic subunits encoded by a multigene family. 'Type-A' legumin subunits contain methionine whereas 'type-B' are methionine-free subunits. Sequencing of two different type A-specific cDNAs, as well as an FPLC/HPLC-based improvement of subunit fractionation and peptide mapping with subsequent partial amino-acid sequencing, permit the assignment of some of the polymorphic legumin subunits to members of the multigene family. Two different type A subunits (A1 and A2) correspond to the two different cDNA clones pVfLa129 (A2) and 165 (A1), but microheterogeneity in the amino-acid sequences indicates that polymorphic variants of both representatives of this type may exist. Two groups of published type B-specific gene sequences (LeB7, and LeB2, LeB4, LeB6, respectively) are represented by two polymorphic subunit fractions (B31, B311, and B41, B411). A seventh clone, LeB3, encodes one of the large legumin subunits that is only a minor component of the legumin seed protein complex. |
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Bibliography: | F30 F |
ISSN: | 0040-5752 1432-2242 |
DOI: | 10.1007/BF00212614 |