Rapid identification of Robinsoniella peoriensis using specific 16S rRNA gene PCR primers

• Published in: Anaerobe Vol. 43; pp. 39 - 42
• Main Authors: Whitehead, Terence R., Anoma, Christelle, McLaughlin, Richard W.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2017
• Subjects: 16S rRNA; Animals; Assay; Bacterial Typing Techniques; Clostridiales - classification; Clostridiales - genetics; Clostridiales - isolation & purification; DNA Primers - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Europe; Feces - microbiology; Gastrointestinal; Humans; Manure - microbiology; PCR; Peoriensis; Phylogeny; Polymerase Chain Reaction - veterinary; RNA, Ribosomal, 16S - genetics; Robinsoniella; Sequence Analysis, DNA; Swine; United States; Assay; Peoriensis; 16S rRNA; Robinsoniella; Gastrointestinal; PCR
• ISSN: 1075-9964; 1095-8274
• DOI: 10.1016/j.anaerobe.2016.11.008

Robinsoniella peoriensis is a Gram-positive, spore-forming anaerobic bacterium initially isolated and characterized from swine manure and feces. Since then strains of this species have been identified from a variety of mammalian and other GI tracts. More recently strains of this species have been isolated from a plethora of human infections. Therefore, it is of great interest to develop methods to rapidly identify this microorganism in the medical and other laboratories. This report describes the use of PCR primers targeting the 16S rRNA gene of R. peoriensis to identify strains of this bacterium. •Robinsoniella peoriensis can be found in a variety of mammalian and other gastrointestinal tracts.•R. peoriensis can also be isolated from a variety of human disease states.•16S rRNA gene PCR primers have been developed for rapid identification of R. peoriensis.