Collagen I confers gamma radiation resistance

• Published in: Applied radiation and isotopes Vol. 71; pp. 71 - 74
• Main Authors: Azorin, E., González-Martínez, P.R., Azorin, J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2012
• Subjects: Animals; Cell Line, Tumor; Collagen; Collagen Type I - pharmacology; Gamma radiation; Gamma Rays; MAP Kinase Signaling System - physiology; MAPK; Neoplasm Invasiveness; Pituitary Neoplasms - radiotherapy; Radiation Tolerance - drug effects; Rats; Thermoluminescent dosimeters; Gamma radiation; Thermoluminescent dosimeters; MAPK; Collagen
• ISSN: 0969-8043; 1872-9800
• DOI: 10.1016/j.apradiso.2012.03.039

The effect of collagen on the response of somatomammotroph tumor cells (GH3) to gamma, radiation therapy was studied in vitro. After incubating confluent GH3 cell monolayers in a serum-free, maintaining medium, either with or without collagen, the monolayers were irradiated with 137Cs, gamma radiation. Collagen reduces cell mortality via ERK1/2 activation, abolishing gamma radiation, cell death, and promotes cell invasion when acting in synergy with collagen and in association with the, MAPK/ERK1/2 signaling pathway activation. The presence of collagen in somatomammotroph tumors, confers resistance to radiation. ► Collagen effect on GH3 cells response to gamma radiation therapy was studied. ► Collagen ERK activation abolishes gamma radiation GH3 cell death. ► Gamma radiation promotes cell invasion and ERK activation in synergy with collagen. ► The presence of collagen in somatomammotroph tumors confers radiotherapy resistance. ► Analysis of tumor surrounding tissue before applying radiotherapy would be advisable.