The Effect of Intestinal Reperfusion on Renal Function and Perfusion

This study examines the effect of intestinal reperfusion injury (IIR) on renal blood flow and relates this temporally to changes in renal ATP levels and renal tubular function. Sprague–Dawley rats underwent 120 min of intestinal ischemia and 60 min of reperfusion (IIR). Renal blood flow was measured...

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Published inThe Journal of surgical research Vol. 64; no. 1; pp. 19 - 25
Main Authors LaNoue, Jr, John L., Turnage, Richard H., Kadesky, Kevin M., Guice, Karen S., Oldham, Keith T., Myers, Stuart I.
Format Journal Article
LanguageEnglish
Published New York, NY Elsevier Inc 15.07.1996
Elsevier
Subjects
Adenosine Triphosphate - metabolism
Animals
Aorta - physiopathology
Biological and medical sciences
Blood Pressure
Gastroenterology. Liver. Pancreas. Abdomen
Intestines - blood supply
Kidney - metabolism
Male
Medical sciences
Microspheres
Other diseases. Semiology
Rats
Rats, Sprague-Dawley
Renal Circulation
Reperfusion Injury - physiopathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Sonography
Doppler ultrasound study
Regional blood flow
Animal model
Renal function
Pathophysiology
Rat
Rodentia
Gut
Cardiovascular disease
Kidney
Vertebrata
Experimental disease
Mammalia
Reperfusion
Ischemia
Animal
Digestive diseases
Intestinal disease
Hemodynamics
ATP
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Summary:This study examines the effect of intestinal reperfusion injury (IIR) on renal blood flow and relates this temporally to changes in renal ATP levels and renal tubular function. Sprague–Dawley rats underwent 120 min of intestinal ischemia and 60 min of reperfusion (IIR). Renal blood flow was measured with radiolabeled microspheres and a Doppler flow probe. Renal dysfunction was quantitated by measuring inulin clearance and fractional excretion of sodium (FENa) in the isolated perfused organ. Renal tissue ATP levels were measured using a luciferase–luciferin assay. Sham-operated animals served as controls (SHAM). Renal blood flow was reduced by >80% in the animals sustaining IIR when compared to baseline measurements (P< 0.05) or SHAM (P< 0.05). Temporally this reduction in renal blood flow was associated with a 25% reduction in tissue ATP levels (P< 0.05). The kidneys of animals sustaining IIR had a significantly greater FENathan did those of controls. These data support the notion that IIR is associated with a profound reduction in renal blood flow which is temporally related to reduced renal tissue ATP levels and renal tubular dysfunction.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-4804
1095-8673
DOI:10.1006/jsre.1996.0300