Purification, chemical characterization and antioxidant activities of a novel polysaccharide from Auricularia polytricha

• Published in: International journal of biological macromolecules Vol. 120; no. Pt A; pp. 1087 - 1092
• Main Authors: Chen, Yiyong, Xue, Yiting
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2018
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Auricularia polytricha; Basidiomycota - chemistry; Biphenyl Compounds - chemistry; Chromatography, Gas; Chromatography, High Pressure Liquid; Free Radical Scavengers - chemistry; Picrates - chemistry; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Purification; Spectroscopy, Fourier Transform Infrared; Superoxides - chemistry; Auricularia polytricha; Polysaccharide; Purification
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.08.160

A novel water-soluble polysaccharide coded APP3a was isolated and purified from Auricularia polytricha by DEAE-52 cellulose and Sephadex G-200 column chromatography. Chemical characterization of APP3a was investigated based on analysis of high performance liquid chromatography (HPLC), gas chromatography (GC), fourier transform infrared spectroscopy (FT-IR) and thermal gravimetric analysis (TGA). Antioxidant activities of APP3a were investigated on the basis of superoxide anion,1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and reducing power. The results showed that APP3a consisted of arabinose, mannose, glucose and galactose in a molar ratio of 1:1.33:1.06:1.23 with an average molecular weight of 21,242 Da. Thermal gravimetric analysis showed that APP3a was relatively stable below 250 °C. APP3a showed the stronger reducing power and stronger scavenging activity against hydroxyl radicals, superoxide radicals and DPPH radicals in a concentration-dependent manner. These findings suggested that the polysaccharide APP3a could be served as a novel antioxidant in functional food.