Effects of water-soluble curcuminoid-rich extract in a solid dispersion form (CRE-SD) on the sperm characteristics, longevity and casein kinase II catalytic subunit alpha protein stability in chilled goat semen

• Published in: Cryobiology Vol. 109; pp. 30 - 36
• Main Authors: Kupthammasan, Navapol, Wittayarat, Manita, Panichayupakaranant, Pharkphoom, Didas, Nutsiwat, Wattanachant, Chaiyawan, Panyaboriban, Saritvich
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.12.2022
• Subjects: Animals; Casein Kinase II - pharmacology; Catalytic Domain; Chilled semen; Cryopreservation - methods; CSNK2A2; Curcuminoid-rich extract; Diarylheptanoids - pharmacology; Female; Goat; Goats; Longevity; Male; Protein Stability; Semen; Semen Preservation - methods; Semen Preservation - veterinary; Sperm; Sperm Motility; Spermatozoa; Water; Goat; Curcuminoid-rich extract; Sperm; Chilled semen; CSNK2A2
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2022.09.008

The present study evaluated the effects of water-soluble curcuminoid-rich extract in a solid dispersion form (CRE-SD) on goat sperm qualities and sperm protein CSNK2A2 expression during liquid storage. Semen was collected from five fertile goats, using an artificial vagina. Ejaculates with a motility above 70% were cooled to 4 °C using TRIS-citric acid-fructose diluent with 10% egg yolk containing various concentrations of CRE-SD (0, 0.1, 1, 10 and 100 μg/mL). Chilled sperm were evaluated for sperm characteristics, casein kinase II catalytic subunit alpha (CSNK2A2) protein level and oxidative status up to 15 days. After 12 days of preservation, sperm motility, viability, acrosomal integrity and mitochondrial activity were significantly higher in the group preserved with 10 μg/mL CRE-SD as compared with the control group. Supplementation of CRE-SD at this concentration was also able to conserve the CSNK2A2 a significantly higher than that in control group until 9 days of cold storage, possibly by reducing oxidative stress. The molecular mass of the sperm CSNK2A2 protein detected in this study was 37 kDa; it was mostly located in the post-acrosomal region, midpiece and flagellum. These results demonstrate the possibility to use the CRE-SD as a natural antioxidant during liquid semen storage in goats. •CRE-SD is easily dissolved in semen extender and has a benefit on chilled sperm quality.•CRE-SD can extend chilled buck sperm longevity to 9 days that is still sufficient for AI.•The 37 kDa-sized CSNK2A2 proteins were detected mainly in sperm flagellum.•CRE-SD can preserve sperm CSNK2A2 during cold storage via oxidative stress reduction.