Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota

• Published in: International journal of biological macromolecules Vol. 226; pp. 1319 - 1331
• Main Authors: Cao, Junhan, Wang, Kai, Li, Nianxu, Zhang, Liping, Qin, Ling, He, Yingying, Wang, Jingfeng, Qu, Changfeng, Miao, Jinlai
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.01.2023
• Subjects: Animals; Cellulose; Cellulose - pharmacology; Constipation; Constipation - chemically induced; Constipation - drug therapy; Dietary Fiber - metabolism; Dietary Fiber - pharmacology; Fatty Acids, Volatile - metabolism; Gastrointestinal Microbiome; Gut microbiota; Humans; IDF; Loperamide; Mice; Neurotransmitter Agents; Phaeophyceae - metabolism; Saccharina japonica; SCFAs; SDF; Saccharina japonica; Gut microbiota; SDF; Cellulose; SCFAs; IDF; Constipation
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2022.11.243

The effects of soluble dietary fiber (SDF) and cellulose (IDF) from Saccharina japonica by-product and their differences in improving constipation were further clarified in the present study. We demonstrated that SDF was mainly made up of d-mannuronic acid and d-mannose while IDF consisted of d-glucose , which is different from other reported dietary fibers of terrestrial plants. In this research, both SDF and IDF improved fecal-related indicators, gastrointestinal transit rate and histological morphology in Lop-induced mice. Moreover, they could increase the level of antioxidant enzymes (SOD and GSH-Px), restore the expression of enteric neurotransmitters, and maintain the function of ZO-1, JAM-1 as well as Occludin. Interestingly, SDF and IDF had a significant up-regulated effect on the proportion of Muribaculacea, Prevotellaceaen and Lachnospiraceae, which are critical to preserving intestinal immune homeostasis. Besides, they promoted the biosynthesis of short-chain fatty acids (SCFAs). The overall index showed that SDF is more effective for constipation due to its better water retention capacity. Thus, they can be used as a safe dietary supplement for the treatment of chronic or occasional constipation in humans. [Display omitted]