Preparation of Ca-alginate-whey protein isolate microcapsules for protection and delivery of L. bulgaricus and L. paracasei

• Published in: International journal of biological macromolecules Vol. 163; pp. 1361 - 1368
• Main Authors: Han, Chenlu, Xiao, Yanju, Liu, Enchao, Su, Zhiwei, Meng, Xianghong, Liu, Bingjie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2020
• Subjects: Alginate; Alginates - chemistry; Calcium - chemistry; Capsules - chemistry; Co-encapsulation; Delivery; Drug Compounding - methods; Freeze Drying - methods; Gastrointestinal; Lactobacillus delbrueckii - chemistry; Lactobacillus paracasei - chemistry; Probiotics; Probiotics - chemistry; Spectroscopy, Fourier Transform Infrared - methods; Thermal properties; Whey protein isolate; Whey Proteins - chemistry; Thermal properties; Probiotics; Co-encapsulation; Alginate; Delivery; Gastrointestinal; Whey protein isolate
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.07.247

To promote the application of probiotics that are beneficial to hosts, calcium-alginate (Ca-Alg) coated whey protein isolate microcapsules were prepared for protection and delivery of L. bulgaricus and L. paracasei. The internal layer was formed with transglutaminase-induced gelation of whey protein isolate (WPI). Sodium alginate (SA) was applied to form outer layer with external Ca2+ gelation method. The microcapsules loaded with probiotics were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC). The results showed that the co-encapsulation efficiency was 90.54% and 84.46% of WPI micro-beads and Ca-Alg-coated microcapsules, respectively. The trehalose was added as cryoprotectant to improve the survival rate of probiotics in freeze-dried Ca-Alg-coated microcapsules from 3% to 41.26%. Ca-Alg-coated microcapsules have regular morphology and intensified structure. The protection of Ca-Alg-coated microcapsule for probiotics was improved under simulated gastrointestinal and thermal conditions. Ca-Alg-WPI microcapsules showed a useful way for protection and delivery of L. bulgaricus and L. paracasei. •The encapsulates were prepared by Ca-alginate-whey protein isolate wall system.•An effective way of delivery probiotics to colon and increasing thermal tolerance•Viability of entrapped cells during freeze-drying was enhanced by trehalose.•Microencapsulated probiotics were stable in simulated gastrointestinal conditions.