Engineering hemagglutinin (H) protein of rinderpest virus into peanut ( Arachis hypogaea L.) as a possible source of vaccine

• Published in: Plant science (Limerick) Vol. 165; no. 1; pp. 77 - 84
• Main Authors: Khandelwal, Abha, Vally, K.J.M., Geetha, N., Venkatachalam, P., Shaila, M.S., Lakshmi Sita, G.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.07.2003; Elsevier Science
• Subjects: Agrobacterium; Arachis hypogaea; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Hemagglutinin protein; Methods. Procedures. Technologies; Peanut; Rinderpest virus; Transformation; Transgenic animals and transgenic plants; Transgenic plants; H, hemagglutinin; Peanut; Transformation; nptII, neomycin phosphotransferase gene; BAP, 6-benzylamniopurine; PCR, polymerase chain reaction; Rinderpest virus; IBA, indole-3-butyric acid; Transgenic plants; Hemagglutinin protein; Hemagglutinin; Arachis hypogaea; Vaccine; Gene expression; Paramyxoviridae; Virus; Paramyxovirinae; Mononegavirales; Leguminosae; Dicotyledones; Animal; Angiospermae; Morbillivirus; Spermatophyta; Transgenic plant; Oil plant (vegetal)
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(03)00145-6

Development of transgenic plants as an expression system for the delivery of recombinant subunit vaccine through fodder would represent the most efficient and economical means of mass immunization of domestic ruminants as well as susceptible wild life. Rinderpest virus causes a highly contagious disease in cattle, buffaloes, sheep, goats and wild ruminants with a high mortality rate. We have developed transgenic peanut ( Arachis hypogea L.) plants expressing the hemagglutinin (H) protein of rinderpest virus. The transgenic peanut plants were produced by Agrobacterium-mediated transformation of shoot apices. The presence and integration of the transgene has been verified by PCR and Southern hybridization. The expression of the hemagglutinin protein was confirmed by immunoblotting analysis employing polyclonal H antibody. The expression of hemagglutinin protein in T 1 generation demonstrates the stable integration of H gene into the plant genome.