Graphene oxide enriched with oxygen-containing groups: on the way to an increase of antioxidant activity and biocompatibility

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 210; p. 112232
• Main Authors: Abdelhalim, Abdelsattar O.E., Meshcheriakov, Anatolii A., Maistrenko, Dmitrii N., Molchanov, Oleg E., Ageev, Sergei V., Ivanova, Daria A., Iamalova, Nailia R., Luttsev, Mikhail D., Vasina, Lubov V., Sharoyko, Vladimir V., Semenov, Konstantin N.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2022
• Subjects: Antioxidants - pharmacology; Biocompatibility; Cytotoxicity; DPPH; Graphene Oxide; Graphite; HEK293 Cells; HSA; Humans; Identification; Oxygen; Biocompatibility; Cytotoxicity; Identification; DPPH; Graphene Oxide; HSA
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2021.112232

The article is dedicated to the comprehensive biocompatibility investigation of synthesised graphene oxide (GO) enriched with oxygen-containing functional groups (⁓85%). GO was synthesised through a modified Hummers and Offeman’s method and characterised using 13C NMR, Raman, and IR spectroscopy, XRD, HRTEM, along with size dimensions and ζ-potentials in aqueous dispersions. Biocompatibility study included tests on haemocompatibility (haemolysis, platelet aggregation, binding to human serum albumin and its esterase activity), antioxidant activity (2,2-diphenyl-1-picrylhydrazyl reaction, NO-radical uptake, Radachlorin photobleaching, photo-induced haemolysis), genotoxicity using DNA comet assay, as well as metabolic activity and proliferation of HEK293 cells. [Display omitted] •Graphene oxide (GO) enriched with oxygen-containing functional groups (85%) was synthesised.•GO has high haemocompatibility and low cyto- and genotoxicity.•GO is able to bind to human serum albumin.•GO possesses pronounced antioxidant properties.