Characterization of an exopolysaccharide with distinct rheological properties from Paenibacillus edaphicus NUST16

• Published in: International journal of biological macromolecules Vol. 105; no. Pt 1; pp. 1 - 8
• Main Authors: Li, Jing, Xu, Haiyang, Chen, Xiangnan, Xu, Linxiang, Cheng, Rui, Zhang, Jianfa, Wang, Shiming
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017
• Subjects: Methylation; Molecular Weight; Paenibacillus - chemistry; Paenibacillus edaphicus; Polysaccharide; Polysaccharides - chemistry; Rheology; Structure; Structure; Rheology; Polysaccharide; Paenibacillus edaphicus
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.06.030

•A newly isolated strain was identified as Paenibacillus edaphicus NUST16.•Molecular structure of the polysaccharide, POS16, was determined.•POS16 shows strong saline-tolerance. Microbial exopolysaccharides (EPSs) have been commercially used for decades owing to their distinct rheological properties. New EPSs with special traits are being explored continuously. The strain Paenibacillus edaphicus NUST16 was isolated in this study. NUST16 produces 12.5g/L EPS after 72h cultivation in shaker flask. Moreover, the purified EPS, POS16, has the molecular weight of 1.2×107Da and consists of five types of glycosides, namely, D-Glc, D-Man, L-Fuc, D-GlcA, and D-Gal. Methylation, NMR, and FT-IR results indicate that three glucosides and three mannosides make up the main chain of POS16 via 1→3 linkage. The Gal(1→3)Fuc(1→3)Fuc(1→ and GlcA(1→ branches link to the C6 positions of two mannosides, respectively. The other mannoside is partially derivatized by the acetyl group at C2 position. Rheological analysis shows that POS16 has typical shear-thinning pattern which fits the Hershel-Bulkley model well. More importantly, POS16 shows ideal saline tolerance as the apparent viscosity keeps high in solutions containing 100g/L salts, such as NaCl, KCl, MgCl2, and CaCl2. Furthermore, POS16 behaves more viscous than xanthan gum and sodium carboxymethylcellulose in divalent ion solutions. The results suggest that POS16 is a potential rheological modulator that can be used in operations where high concentrations of salts exist.