Induction of cyclic electron flow around photosystem I during heat stress in grape leaves

• Published in: Plant science (Limerick) Vol. 256; pp. 65 - 71
• Main Authors: Sun, Yongjiang, Geng, Qingwei, Du, Yuanpeng, Yang, Xinghong, Zhai, Heng
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.03.2017
• Subjects: Adaptation, Physiological; Chlorophyll - metabolism; Cyclic electron flow; Electron Transport; Fluorescence; Grape; Heat stress; Hot Temperature; Light; NADPH Dehydrogenase - metabolism; Oxidation-Reduction; Photoinhibition; Photosynthesis; Photosystem I Protein Complex - metabolism; Photosystem II Protein Complex - metabolism; Plant Leaves - metabolism; Species Specificity; Stress, Physiological; Vitis - classification; Vitis - metabolism; Photoinhibition; Cyclic electron flow; Heat stress; Grape
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2016.12.004

•We studied the functions of PSI and PSII in leaves at different high temperatures.•The stimulation of CEF under heat stress contributes to the generation of a proton gradient.•The heat stress-induced NDH-dependent CEF varied between different heat tolerant grape cultivators. Photosystem II (PSII) in plants is susceptible to high temperatures. The cyclic electron flow (CEF) around PSI is thought to protect both PSII and PSI from photodamage. However, the underlying physiological mechanisms of the photosynthetic electron transport process and the role of CEF in grape at high temperatures remain unclear. To investigate this issue, we examined the responses of PSII energy distribution, the P700 redox state and CEF to high temperatures in grape leaves. After exposing ‘Cabernet Sauvignon’ leaves to various temperatures (25, 30, 35, 40 and 45°C) in the light (600μmol photons m−2s−1) for 4h, the maximum quantum yield of PSII (Fv/Fm) significantly decreased at high temperatures (40 and 45°C), while the maximum photo-oxidizable P700 (Pm) was not affected. As the temperature increased, higher initial rates of increase in post-illumination Chl fluorescence were detected, which were accompanied by an increase in high energy state quenching (qE). The chloroplast NAD(P)H dehydrogenase-dependent CEF (NDH-dependent CEF) activities were different among grape cultivators. ‘Gold Finger’ with greater susceptibility to photoinhibition, exhibited lower NDH-dependent CEF activities under acute heat stress than a more heat tolerant ‘Cabernet Sauvignon’. These results suggest that overclosure of PSII reaction centers at high temperature resulted in the photoinhibition of PSII, while the stimulation of CEF in grape played an important role in the photoprotection of PSII and PSI at high temperatures through contributing to the generation of a proton gradient.