Response of tomato (Solanum L. section Lycopersicon Mill.) germplasm to begomovirus inoculation under controlled and field conditions

• Published in: Genetic resources and crop evolution Vol. 61; no. 2; pp. 435 - 450
• Main Authors: Aguilera, Jorge González, Hurtado, Francisco Dueñas, Sobrinho, Roberto Ramos, de Souza Almeida, Victor, Tavares, Sheila S, Nick, Carlos, Soares, Marcelo Oliveira, Xavier, Cesar A. Diniz, de Freitas, Renata Dias, Gil, Martha Alvarez, Zerbini, Francisco Murilo, da Silva, Derly J. Henriques
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.02.2014; Springer Netherlands; Springer Nature B.V
• Subjects: Agriculture; alleles; Begomovirus; Biomedical and Life Sciences; Correlation analysis; Cultivars; Germplasm; greenhouses; Heterozygosity; Hybridization; Infections; Inoculation; Life Sciences; Lycopersicon; Lycopersicon esculentum; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Plant viruses; Research Article; Solanum; Solanum lycopersicum; Solanum peruvianum; Statistical analysis; Tomatoes; vegetable crops; Viral infections; virus replication; Brazil; Resistance; Germplasm; 2; Genetic variability; 3
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-013-0048-3

Ninety-five tomato accessions belonging to the Vegetable Crops Germplasm Bank of the Federal University of Viçosa (BGH-UFV) were evaluated in a protected environment (biolistic inoculation under greenhouse conditions) and in the field (natural infection) for resistance to bipartite begomoviruses present in Brazil. Plants were assessed by the visual evaluation of symptoms, and viral infection was confirmed by non-radioactive molecular hybridization. Univariate statistical analyses and correlations were made between the traits. High and significant correlations were found between the percentages of visual symptoms and of viral replication, with values of 0.67 in the protected environment and 0.60 in the field. We concluded that under the protected environment, the accessions BGH-2144, BGH-2150, BGH-6878 and BGH-6881 displayed resistance to infection. Under field conditions with natural infection, the best results were obtained for the accessions BGH-2080 and BGH-6881. Only the BGH-6881 (Solanum peruvianum L.) accession excelled in both conditions; this resistance was attributed to the presence of the Ty-2 and Ty-3 resistance gene alleles in heterozygosity. In general, all of the accessions selected in both experimental conditions can serve as sources for the development of cultivars tolerant or resistant to the bipartite begomoviruses present in Brazil.