Optimization of microwave-assisted extraction of polysaccharide from Psidium guajava L. fruits

• Published in: International journal of biological macromolecules Vol. 91; pp. 227 - 232
• Main Authors: Amutha Gnana Arasi, Michael Antony Samy, Gopal Rao, Manchineela, Bagyalakshmi, Janardanan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2016
• Subjects: Central composite design; Extraction; Fruit - chemistry; Microwave; Microwaves; Optimization; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Psidium - chemistry; Response surface methodology; Microwave; Extraction; Central composite design; Response surface methodology; Optimization
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2016.05.039

This study deals with the optimization of microwave assisted extraction of polysaccharide from Psidium guajava L. fruit using Response surface methodology. To evaluate the effect of three independent variables, Water to plant material ratio, microwave power used for extraction and Irradiation time, central composite design has been employed. The yield is considered as dependent variable. The design model estimated the optimum yield of 6.81677% at 200W microwave power level, 3:1 water to plant material ratio and 20min of irradiation time. Three factors three levels Central composite design coupled with RSM was used to model the extraction process. ANOVA was performed to find the significance of the model. The polysaccharide extracted using microwave assisted extraction process was analyzed using FTIR Spectroscopy.