A new strategy to improve Ganoderma polysaccharides production by symbiotic fungi elicitors through activating the biosynthetic pathway

• Published in: International journal of biological macromolecules Vol. 235; p. 123798
• Main Authors: Xu, Juan, Yan, Xiaoyun, Jia, Xumei, Wang, Ying, Xu, Haishun, Yu, Haizheng, He, Liang, Zheng, BingSong, Wu, Xueqian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.04.2023
• Subjects: Biosynthetic Pathways; Biotic eliciting; Ganoderma; Ganoderma lucidum polysaccharides; Humans; Male; Monosaccharides; Polysaccharides; Reishi; Soil symbiotic fungi; Soil symbiotic fungi; Biotic eliciting; Ganoderma lucidum polysaccharides
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.123798

Ganoderma lucidum polysaccharides (GLP) attract growing attention due to their remarkable bioactivities, but the low content in raw materials remains a bottleneck severely restricting their application. We previously found a higher polysaccharides accumulation in Ganoderma lucidum cultured in continuous cropping soil, and soil symbiotic fungi are presumed as the key among many factors. Herein, 33 symbiotic fungi were isolated from the soil, and fungal elicitors were prepared to investigate their biotic eliciting effect on GLP biosynthesis. Most elicitors were found to significantly improve GLP production, among which the NO.16 molecularly identified as Penicillium citrinum, exhibited the optimum eliciting effect with GLP yield increasing by 3.4 times. Differences in the biosynthetic pathway genes expressions and the monosaccharide components of GLP were further analyzed. The transcriptions of the main genes of GLP biosynthetic pathway were up-regulated under PCE treatments, suggesting it improves GLP production by activating transcriptions of the biosynthetic pathway genes. Moreover, PCE eliciting significantly altered the monosaccharide compositions of GLP with Gal, Man, GalA, GlcA, and Fuc increasing by 8.17 %, 5.68 %, 5.41 %, 2.66 %, and 1.51 % respectively, but Glc decreased by 23.43 %, which may result in the activity change. It can serve as a new strategy to improve GLP production. [Display omitted] •33 symbiotic fungi were isolated from the continuous cropping soil of G. lucidum.•Most fungal elicitors made from the symbiotic fungi notably enhanced GLP production•The optimum elicitor PCE increased the GLP yield by 3.4 times.•PCE improved GLP production by activating the biosynthetic pathway genes expression.•PCE could also altered the monosaccharide compositions of GLP.