Structural, physical characteristics and biological activities assessment of scleroglucan from a local strain Athelia rolfsii TEMG

• Published in: International journal of biological macromolecules Vol. 163; pp. 1196 - 1207
• Main Authors: Elsehemy, Islam A., Noor El Deen, Azza M., Awad, Hassan M., Kalaba, Mohamed H., Moghannem, Saad A., Tolba, Ibrahim H., Farid, Mohamed A.M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2020
• Subjects: Antiviral activity against H5N1 and HSV-1; Athelia rolfsii TEMG; Basidiomycota - chemistry; Basidiomycota - classification; Basidiomycota - genetics; Cell Survival - drug effects; Chemical Phenomena; Exopolysaccharide characterization; Glucans - chemistry; Glucans - pharmacology; Humans; Molecular Structure; Molecular Weight; Monosaccharides - chemistry; Polysaccharides - chemistry; Polysaccharides - pharmacology; Rheology; Scleroglucan; Structure-Activity Relationship; Exopolysaccharide characterization; Antiviral activity against H5N1 and HSV-1; Athelia rolfsii TEMG; Scleroglucan
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.06.272

Athelia rolfsii TEMG (MH 236106) an exopolysaccharide (EPS) producing fungal strain was isolated and identified. Extraction, purification, characterization, antimicrobial, antioxidant, antiviral and antitumor activities of the polysaccharide were investigated. It characterized as a homopolysaccharide of glucose with a molecular weight of 345.622 kDa. The identification of the polysaccharide was conducted using scanning electron microscopy, energy dispersive X-ray analysis, 1H and 13C NMR spectra. The existence of β-1,3 and β-1,6 linkages suggests that EPS could be scleroglucan. The purified scleroglucan showed considerable antibacterial and antioxidant activities. The results indicated that, there was no cytotoxicity on normal cell (W138) and no effect on tumor cell lines including HepG2 and PC3 showing IC50 of 5096.83, 5885.80 and 4803.90 μg/mL, respectively. The results showed also that Sclg could reduce the cytopathic effect by 50% (EC50) at 15 and 50 μg/mL of herpes simplex virus type-1 (HSV-1) and influenza virus (H5N1), respectively. •Scleroglucan an exopolysaccharide by Athelia rolfsii TEMG (MH 236106)•Molecular identification of Athelia rolfsii TEMG•Extraction and purification of scleroglucan•Electron microscopy and energy dispersive X-ray, HPLC, UV, NMR confirmation of scleroglucan•Antiviral activity against influenza virus H5N1 and HSV-1