Bio-inspired synthesis of chitosan/copper oxide nanocomposite using rutin and their anti-proliferative activity in human lung cancer cells

• Published in: International journal of biological macromolecules Vol. 141; pp. 476 - 483
• Main Authors: Bharathi, Devaraj, Ranjithkumar, R., Chandarshekar, B., Bhuvaneshwari, V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2019
• Subjects: A549 cancer cells; A549 Cells; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Chitosan; Chitosan - chemistry; Chitosan - pharmacology; Copper - chemistry; Copper - pharmacology; Copper oxide nanocomposite; Humans; Lung Neoplasms - drug therapy; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Nanocomposites - chemistry; Nanocomposites - therapeutic use; Rutin; Rutin - chemistry; Rutin - pharmacology; Copper oxide nanocomposite; Chitosan; A549 cancer cells; Rutin
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.08.235

Polymer functionalized metal oxide nanocomposites are great interest due to wide range of application, especially in nanomedicine. The present study reports an eco-friendly bio-inspired synthesis of chitosan/copper oxide (CS-CuO) nanocomposite for the first time using rutin. The bio-synthesized CS-CuO nanocomposite was characterized using UV–Visible spectroscopy, FE-SEM, EDS, TEM, XRD and FTIR analyses. FE-SEM and TEM images revealed the synthesized CS-CuO nanocomposite having spherical shaped structure with an average size of 10–30 nm. EDS analysis confirmed the elements present in synthesized CS-CuO nanocomposite. FTIR studies revealed the role of rutin and chitosan for reduction, capping and synthesis of CS-CuO nanocomposite from the precursor copper salt. The XRD analysis revealed monoclinic structure of CS-CuO nanocomposite. Anti-proliferative activity of the CS-CuO nanocomposite was evaluated in human lung cancer cell line A549. Synthesized CS-CuO nanocomposite showed concentration-depended anti-proliferative activity against A549 cancer cells and their IC50 value was found to be 20 ± 0.50 μg/mL. Furthermore, synthesized nanocomposite induce apoptosis in treated A549 cancer cells assayed by AO/EtBr fluorescent staining method. In conclusion, the synthesized CS-CuO nanocomposite using rutin can be used as a potential anticancer agent in biomedical and clinical sectors. •Chitosan/copper oxide (CS-CuO) nanocomposite was synthesized for the first time using rutin.•Prepared nanocomposite was characterized by UV-Vis, FE-SEM, TEM, XRD and FT-IR analyses.•Prepared nanocomposite showed significant anti-proliferative activity in A549 cells.