Exon Mapping by Fiber-FISH or LR-PCR

• Published in: Genomics (San Diego, Calif.) Vol. 38; no. 3; pp. 277 - 282
• Main Authors: Florijn, Ralph J., Ruke, Frans M.van de, Vrolijk, Hans, Blonden, Lau A.J., Hofker, Marten H., Dunnen, Johan T.den, Tanke, Hans J., Ommen, Gert-Jan B.van, Raap, Anton K.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.12.1996; Elsevier
• Subjects: Biological and medical sciences; Chromosome Mapping - methods; Classical genetics, quantitative genetics, hybrids; Cosmids - genetics; DNA, Complementary - genetics; DNA, Complementary - ultrastructure; DNA, Recombinant - genetics; DNA, Recombinant - ultrastructure; Dystrophin - genetics; Exons - genetics; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Humans; In Situ Hybridization, Fluorescence - methods; Methods, theories and miscellaneous; Microscopy, Fluorescence; Muscular Dystrophies - genetics; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Sequence Tagged Sites; Genetic mapping; Human; Polymerase chain reaction; Exon; Contig; Complementary DNA; Genetic marker; Chromosome; Expressed sequence tag; Sequence tagged site; Physical map
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1996.0629

In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of ≥200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.