Exon Mapping by Fiber-FISH or LR-PCR

In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of ≥200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two indepen...

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Published inGenomics (San Diego, Calif.) Vol. 38; no. 3; pp. 277 - 282
Main Authors Florijn, Ralph J., Ruke, Frans M.van de, Vrolijk, Hans, Blonden, Lau A.J., Hofker, Marten H., Dunnen, Johan T.den, Tanke, Hans J., Ommen, Gert-Jan B.van, Raap, Anton K.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.12.1996
Elsevier
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Summary:In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of ≥200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.
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ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0629