Automatic Compensation of Total Phase Aberrations in Digital Holographic Biological Imaging
Digital holographic microscopy has heen a powerful metrological technique for phase-contrast imaging. However inherent phase aberrations always exist and degrade the quality of the phase-contrast images. A surface fitting method based on an improved mathematic model is proposed, which ran he used to...
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Published in | Chinese physics letters Vol. 28; no. 11; pp. 114209 - 1-114209-4 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
IOP Publishing
01.11.2011
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Subjects | |
Online Access | Get full text |
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Summary: | Digital holographic microscopy has heen a powerful metrological technique for phase-contrast imaging. However inherent phase aberrations always exist and degrade the quality of the phase-contrast images. A surface fitting method based on an improved mathematic model is proposed, which ran he used to remove the phase aberrations without any pre-knowledge of the setup or manual operation. The improved mathematic model includes not only the usual terms but also the cross terms and the high order terms to describe the phase aberrations with high accuracy Meanwhile, a non-iterative algorithm is used to solve the parametersand thus less computational load is imposed. The proposed method is applied to the live imaging of cells. The experimental results verify its validity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0256-307X 1741-3540 |
DOI: | 10.1088/0256-307X/28/11/114209 |