Antioxidative effect of melatonin on cryopreserved ovarian tissue in mice

• Published in: Cryobiology Vol. 96; pp. 99 - 105
• Main Authors: Liu, Xiao Chao, Sun, Tie Cheng, Li, Hui Ying, Si, Li Na, Wei, Meng, Chen, Zhi Hong, Cheng, Lu Yang, Yang, Song He
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.10.2020
• Subjects: Animals; Antioxidants - pharmacology; Cryopreservation; Cryopreservation - methods; Female; Glutathione Peroxidase - metabolism; Humans; Malondialdehyde; Melatonin; Melatonin - pharmacology; Mice; Ovarian tissues; Oxidative damage; Oxidative Stress; Melatonin; Cryopreservation; Oxidative damage; Ovarian tissues
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2020.07.010

Cryopreservation of ovarian tissues (OTs) has become the most effective way to preserve the fertility of female cancer patients. However, cryopreservation of OTs is still relatively at an experimental stage. The aim of study is to examine the effect of melatonin (MTL) on cryopreserved-thawed OTs. Fragments of OTs were cryopreserved in medium containing different concentrations (0 mM, 0.001 mM, 0.01 mM, 0.1 mM and 1 mM) of MLT. The endogenous enzymes (GSH-PX, GSH, SOD, CAT and T-AOC), MDA and ROS levels were all evaluated after cryopreservation. Our results showed that the 0.1 mM of MLT significantly improved the survival and diameter of follicles (P < 0.001). Meanwhile, the antioxidant enzymes activities (including GSH-PX, GSH, SOD, CAT and T-AOC) were enhanced and MDA content were significantly decreased in 0.1 mM of MLT group compared to other groups (P < 0.001). Additionally, compared to the control group, MTL of 0.1 mM resulted in a significantly lower ROS level. In conclusion, MLT protects the quality of cryopreserved OTs by decreasing oxidative stress level and the optimal concentration is 0.1 mM.