Structural identification and immunostimulating activity of a Laminaria japonica polysaccharide
In the present study, a new water-soluble polysaccharide (LJP-11) was obtained from Laminaria japonica by anion exchange DEAE-cellulose chromatography and Sephacryl S-500 chromatography. The average molecular weight of this polysaccharide was estimated to be about 2.89×106Da by high performance liqu...
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Published in | International journal of biological macromolecules Vol. 78; pp. 429 - 438 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.07.2015
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Subjects | |
Online Access | Get full text |
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Summary: | In the present study, a new water-soluble polysaccharide (LJP-11) was obtained from Laminaria japonica by anion exchange DEAE-cellulose chromatography and Sephacryl S-500 chromatography. The average molecular weight of this polysaccharide was estimated to be about 2.89×106Da by high performance liquid chromatography system. Gas chromatography showed that LJP-11 was composed of arabinose, mannose and glucose in a molar ratio of 1.0:1.16:6.33. LJP-11 contains a long backbone consisting of (1→4)-β-d-GlcpAc, (1→4)-α-d-Glcp, (1→6)-β-d-Glcp and (1→3,6)-α-d-Manp. The 1-linked β-l-Araf was linked to the C-6 of (1→3)-α-d-Manp and the sulfate group was attached to the C-4 of (1→6)-β-d-Glcp. Pharmacological tests displayed that LJP-11 can stimulate macrophages to release NO, IL-6, TNF-α and IL-10 as well as the up-regulation of their gene expressions, indicating LJP-11 has beneficial effects on immunostimulation. Moreover, LJP-11 exhibited positive effects on the translocation of NF-κB p65 from cytoplasm to nucleus and the phosphorylation of IκBα, ERK1/2, JNK1/2 and P38 in macrophages. These results suggested that the activation of MAPK and NF-κB signaling pathways is one of the mechanisms responsible for the immunostimulating activity of LJP-11. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-8130 1879-0003 |
DOI: | 10.1016/j.ijbiomac.2015.04.047 |