CASP3 protein expression by flow cytometry in Down’s syndrome subjects

Down’s syndrome (DS), the most common chromosomal disorder, is caused by 21 trisomy and is featured by intellectual disability. Subjects with DS can develop some traits of Alzheimer disease (AD) at an earlier age than subjects without trisomy 21. Apoptosis is a programmed cell death process under bo...

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Published inHuman cell : official journal of Human Cell Research Society Vol. 27; no. 1; pp. 43 - 45
Main Authors Salemi, Michele, Condorelli, Rosita A., Romano, Corrado, Concetta, Barone, Romano, Carmelo, Salluzzo, Maria Grazia, Bosco, Paolo, Calogero, Aldo E.
Format Journal Article
LanguageEnglish
Published Tokyo Springer Japan 01.01.2014
Subjects
Adult
Alzheimer Disease - genetics
Alzheimer Disease - pathology
Apoptosis - genetics
Biomedical and Life Sciences
Caspase 3 - genetics
Caspase 3 - metabolism
Cell Biology
Cells, Cultured
Down Syndrome - genetics
Down Syndrome - pathology
Female
Fibroblasts - metabolism
Fibroblasts - pathology
Flow Cytometry
Gene Expression - genetics
Gynecology
Humans
Letter to the Editor
Life Sciences
Male
Middle Aged
Oncology
Reproductive Medicine
Stem Cells
Surgery
Up-Regulation
Fibroblasts
Flow cytometry
CASP3 gene
Down syndrome
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Summary:Down’s syndrome (DS), the most common chromosomal disorder, is caused by 21 trisomy and is featured by intellectual disability. Subjects with DS can develop some traits of Alzheimer disease (AD) at an earlier age than subjects without trisomy 21. Apoptosis is a programmed cell death process under both normal physiological and pathological conditions. Caspase-3 (CASP3) plays an important role in neuronal death during nervous system development and under certain pathological conditions. Furthermore, in vitro and in vivo studies report elevated expression and activation of CASP3 in models of AD. On this account, the expression of CASP3 gene was evaluated in cultures of fibroblasts of DS and normal subjects by flow cytometry. CASP3 protein was up-regulated in fibroblasts of DS. The data obtained from this study strengthen the hypothesis that the over-expression of CASP3 gene could have a role in the activation of the apoptotic pathways acting in the neurodegenerative processes in DS.
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ISSN:1749-0774
1749-0774
DOI:10.1007/s13577-013-0071-x