Evaluation of the production of exopolysaccharide by plant growth promoting yeast Rhodotorula sp. strain CAH2 under abiotic stress conditions

• Published in: International journal of biological macromolecules Vol. 121; pp. 55 - 62
• Main Authors: Silambarasan, Sivagnanam, Logeswari, Peter, Cornejo, Pablo, Kannan, Velu Rajesh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2019
• Subjects: Abiotic stresses; Exopolysaccharide; Fungal Polysaccharides - biosynthesis; Fungal Polysaccharides - chemistry; Plant Development; Plant growth promoting traits; Plants - microbiology; Rhodotorula - metabolism; Rhodotorula - physiology; Rhodotorula sp. strain CAH2; Stress, Physiological; Abiotic stresses; Plant growth promoting traits; Exopolysaccharide; Rhodotorula sp. strain CAH2
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2018.10.016

The capability of plant growth promoting microbes to survive under abiotic stresses has important significance for improving plant growth and productivity. Among the various plant growth promoting biomolecules produced by microbes, exopolysaccharide (EPS) help microbes to survive in inhospitable environments and endure environmental stressful conditions. In the present study, a yeast strain CAH2 was isolated from Beta vulgaris rhizosphere soil and identified as Rhodotorula sp., based on the partial 18S rRNA gene sequence analysis. Rhodotorula sp. strain CAH2 was found to tolerate higher concentrations of Al (6 mM), NaCl (150 mM) and PEG-6000 (15%, w/v). The strain CAH2 was shown to produce 7.5 g L−1 of EPS in the production medium with sucrose and yeast extract as a carbon and nitrogen sources, respectively. The EPS yield was increased constantly with increasing concentrations of Al, NaCl and PEG-6000. The structural feature of EPS studied through FT-IR and NMR spectral analysis confirmed the presence of glucose, mannose and galactose. The yeast strain CAH2 was produced multiple plant growth promoting traits in the presence and absence of abiotic stresses. Finally, these results indicate that the production of EPS could be safeguard the plant growth promoting Rhodotorula sp. strain CAH2 from unfavourable environmental conditions. •A Rhodotorula sp. strain CAH2 was highly resistant to abiotic stresses.•Strain CAH2 produced 7.5 g L−1 of EPS under normal condition.•Increased EPS production was observed with abiotic stress condition.•EPS was found to be composed of glucose, mannose and galactose.•Strain CAH2 had plant growth promoting traits were sustained under abiotic stresses.