The WUSCHEL-related homeobox transcription factor MtWOX9-1 stimulates somatic embryogenesis in Medicago truncatula
Somatic embryogenesis is widely used in plant biotechnology, but regulatory networks underlying this process are not fully understood. This process is regulated by many protein and hormonal factors, among which WUSCHEL-related homeobox proteins are believed to play key roles. In this study, we aimed...
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Published in | Plant cell, tissue and organ culture Vol. 138; no. 3; pp. 517 - 527 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.09.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Somatic embryogenesis is widely used in plant biotechnology, but regulatory networks underlying this process are not fully understood. This process is regulated by many protein and hormonal factors, among which WUSCHEL-related homeobox proteins are believed to play key roles. In this study, we aimed to identify the role of
Medicago truncatula WOX9
-
1
(
MtWOX9
-
1
), an
Arabidopsis thaliana WOX9
(
STIMPY
) homolog, in somatic embryogenesis. We demonstrated that the
MtWOX9
-
1
promoter is active in somatic embryos, and overexpression of
MtWOX9
-
1
leads to the increase in embryogenic capacity, as well as to the changes in expression levels of two MADS-box genes (
AGL15
and
AGL8
homologs), associated with embryogenesis. The data obtained suggest that stimulation of
MtWOX9
-
1
expression may have positive impact on plant biotechnology in improving the transformation and regeneration capacity of recalcitrant plants.
Key message
MtWOX9-1 participates in somatic embryogenesis and can stimulate this process in tissue culture changing the expression level of different embryogenesis-associated genes. |
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ISSN: | 0167-6857 1573-5044 |
DOI: | 10.1007/s11240-019-01648-w |