A signal R3-type, CAPRICE-like MYB transcription factor from Dendrobium nobile controls trichome and root-hair development in Arabidopsis

• Published in: Plant science (Limerick) Vol. 337; p. 111878
• Main Authors: Xu, Wenqi, Nyamaharo, Kundai Chelsea, Huang, Yinshuai, Mei, Jun, Guo, Wanli, Ke, Liping, Sun, Yuqiang
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.12.2023
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; CAPRICE like MYB；Epidermal cell; Dendrobium - genetics; Dendrobium - growth & development; Dendrobium - metabolism; Dendrobium nobile; Gene Expression Regulation, Plant; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - growth & development; Plant Roots - metabolism; Plants, Genetically Modified - genetics; Root hair; Transcription Factors - genetics; Transcription Factors - metabolism; Trichome; Trichomes - genetics; Trichomes - growth & development; Trichomes - metabolism; CAPRICE like MYB；Epidermal cell; Dendrobium nobile; Trichome; Root hair
• ISSN: 0168-9452; 1873-2259; 1873-2259
• DOI: 10.1016/j.plantsci.2023.111878

The CAPRICE-like MYB transcription factors with R3 MYB motif play a central role in regulating trichome and root-hair development in plants. We identified the homologous gene of ENHANCER OF TRY AND CPC (ETC) in Arabidopsis from Dendrobium nobile Lindl with full cDNA sequence and genomic sequence (CAPRICE-LIKE MYB, DnCPL and DngCPL) respectively. Phylogenic analyses revealed a close relationship of CAPRICE-like MYB TFs between D. nobile and A. thaliana. Promoter analysis indicated that DnCPL is specifically expressed in trichome basal cells of leaf epidermis and root hairs. Overexpression of DnCPL results in the suppression of trichome formation and overproduction of root hairs. In transgenic plants overexpressing DnCPL and DngCPL, trichome formation was inhibited, moreover, no trichomes were observed in tissues of aerial parts, and root-hair differentiation was significantly enhanced by strongly repressing endogenous genes of AtCPC, AtTCL1, and AtTCL2 expression, thereby enhancing AtTRY expression. The DnCPL RNAi plants formed fewer lateral roots with a corresponding change in AtCPC, AtTCL1 and AtTCL2 expression. These results suggest that Dendrobium and Arabidopsis partially use similar transcription factors for epidermal cell differentiation and the CPC-like R3 MYB, DnCPL, may be a key common regulator of plant trichome and root-hair development. The results also provided genes and means of regulation to improve the survival ratio of artificially cultivated Dendrobium with more lateral roots. •DnCPL from Dendrobium nobile is a CAPRICE-like MYB TF in regulating trichome and root-hair development.•Overexpressing DnCPL reduces leaf trichomes and increases laterial roots in Arabidopsis.•DnCPL affects endogenous genes related to epidermal cell differentiation to regulate trichome and root hair formation.