Gold nanoflowers synthesized using Acanthopanacis cortex extract inhibit inflammatory mediators in LPS-induced RAW264.7 macrophages via NF-κB and AP-1 pathways

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 162; pp. 398 - 404
• Main Authors: Ahn, Sungeun, Singh, Priyanka, Jang, Mi, Kim, Yu-Jin, Castro-Aceituno, Verónica, Simu, Shakina Yesmin, Kim, Yeon Ju, Yang, Deok-Chun
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2018
• Subjects: Acanthopanacis cortex; Gold nanoflowers; Green synthesis; Inflammation; Green synthesis; Inflammation; Gold nanoflowers; Acanthopanacis cortex
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2017.11.037

[Display omitted] •Acanthopanacis-AuNPs suppressed NF-κB and AP-1 through phosphorylation of MAPKs signaling in RAW264.7 cells.•It also inhibited the LPS-induced production of iNOS and COX-2 in the RAW 264.7 cells.•Acanthopanacis-AuNPs may be able to inhibit the inflammatory diseases. We reported the rapid synthesis (<8s) of gold nanoparticles at room temperature using Acanthopanacis cortex extract (A-AuNPs). We characterized the A-AuNPs using several analytical techniques and found that nano-flower type A-AuNPs, which are known to possess a coarse surface with a high surface to volume ratio, conferring these particles with high binding capacity for various biological molecules. After confirming the stability of the nanoparticles, we investigated the anti-inflammatory effect of A-AuNPs in LPS-stimulated RAW264.7 cells. These nanoparticles inhibited LPS-induced iNOS and COX-2 protein as well as gene expression level, along with reduction of NO and PGE2 production. Furthermore, we observed that the A-AuNPs inhibited translocation of NF-κB and AP-1 through phosphorylation of MAPK signaling by western blot analysis. In summary, we synthesized gold nanoflowers in an economical and eco-friendly way using Acanthopanacis cortex extract and the resultant flower-like A-AuNPs had anti-inflammatory activity, highlighting their potential as therapeutic candidates for suppression of inflammatory-mediated diseases.