MicroRNA-6807-3p promotes the tumorigenesis of glioma by targeting downstream DACH1

• Published in: Brain research Vol. 1708; pp. 47 - 57
• Main Authors: Lu, Gui-Feng, Geng, Fei, Xiao, Zhi, Chen, Yuan-Shou, Han, Yong, You, Chun-Yue, Gong, Nan-Ling, Xie, Ze-Mei, Pan, Min
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2019
• Subjects: DACH1; Glioma; Migration; MiR-6807-3p; Proliferation; DACH1; Proliferation; Glioma; MiR-6807-3p; Migration
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2018.12.008

•Reversed expression patterns of miR-6807-3p and DACH1 were observed in glioma tissues.•miR-6807-3p modulates glioma progression by targeting DACH1.•DACH1 serves as a potential biomarker for the prognosis of glioma.•MiR-6807-3p functions as an oncogene in a miR-217-5p-independent manner. Accumulated evidence reveals that microRNAs play vital roles in various tumors, including gliomas. MiRNAs have been shown to participate in multiple cellular functions, including cell proliferation, migration and apoptosis. Here, we investigate the potential role of a novel miRNA, miR-6807-3p, in glioma. A quantitative reverse transcription-polymerase chain reaction (RT-qPCR) and western blot were applied to detect the expression of miR-6807-3p and its target molecule in glioma specimens and cultured cells. The direct targets of miR-6807-3p were predicted by bioinformatics software and were further verified by a luciferase reporter assay. The effects of miR-6807-3p on glioma cell proliferation, migration, cell apoptosis and the cell cycle of glioma cells were analyzed by the Cell-Counting Kit-8 (CCK-8) assay, a cell migration assay and flow cytometry assays. MiR-6807-3p was found to promote tumor growth and migration and inhibits apoptosis and cell cycle arrest in vitro, thus playing a tumor oncogenic role in the progression of glioma. Expression levels of miR-6807-3p were greatly upregulated in glioma specimens, and dachshund homolog 1 (DACH1) was ascertained as a direct target of miR-6807-3p, modulated by the expression of miR-6807-3p in glioma cells. Aberrant expression of DACH1 was associated with the clinical survival of glioma patients. Furthermore, overexpression of DACH1 rescued the promotive effects of miR-6807-3p in glioma. Based on these findings, a novel miR-6807-3p may act as a glioma enhancer by targeting DACH1.