Genotoxic effects of two different classes of insecticide in developing chick embryos

• Published in: Toxicological and environmental chemistry Vol. 95; no. 6; pp. 992 - 1005
• Main Authors: Uggini, Gowri K., Suresh, Balakrishnan
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 01.07.2013; Taylor & Francis Ltd
• Subjects: Assaying; Bioassay; chick embryo; Chicks; chlorpyrifos; combination insecticide; comet assay; cypermethrin; Cytotoxicity; Damage detection; Embryos; embryotoxic; Erythrocytes; Genotoxicity; in ovo injection; Insecticides; micronucleus test; Poultry; spinosad; Toxicology
• ISSN: 0277-2248; 1029-0486
• DOI: 10.1080/02772248.2013.828888

Pesticides provide considerable protection against pest population; however, rampant accumulation of these chemicals into varied habitats across the globe necessitates the need for a careful screening of each chemical due to toxic manifestations. In the current study, the genotoxic potential of two different classes of commercial insecticides - chlorpyrifos and cypermethrin combination and Spinosad, a naturalyte were compared. Rhode Island Red chick embryos were exposed to different doses of either of these insecticides individually, by in ovo treatment. Genotoxicity was then evaluated through micronucleus (MN) test and Comet assay. The combination insecticide exposure at low doses of 0.05 and 0.1 μg/egg induced DNA damage as evidenced by an increased tail moment in the Comet assay. Further, the presence of micronucleated erythrocytes and also various abnormal cells including dacryocytes, microcytes, erythroplastids, squashed/notched nuclei, and spindle-shaped erythrocytes in the blood smear consolidates indicate the presence of insecticide-induced genotoxicity. Spinosad, however, was found only mildly genotoxic but at a high dose of 1.5 mg/egg. The results indicate that usage of naturalyte insecticide may be a better option to minimize the harmful effects of chemical insecticides.