Localization of the Human β-Catenin Gene (CTNNB1) to 3p21: A Region Implicated in Tumor Development

• Published in: Genomics (San Diego, Calif.) Vol. 23; no. 1; pp. 272 - 274
• Main Authors: Kraus, Cornelia, Liehr, Thomas, Hülsken, Jörg, Behrens, Jürgen, Birchmeier, Walter, Grzeschik, Karl-Heinz, Ballhausen, Wolfgang G.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1994; Elsevier
• Subjects: Adenomatous Polyposis Coli Protein; Animals; Armadillo Domain Proteins; Base Sequence; beta Catenin; Biological and medical sciences; Chromosome Mapping; Chromosomes, Human, Pair 3; Classical genetics, quantitative genetics, hybrids; Cytoskeletal Proteins - genetics; Cytoskeletal Proteins - physiology; Drosophila melanogaster - genetics; Drosophila Proteins; Fundamental and applied biological sciences. Psychology; Genes; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Mice; Molecular Sequence Data; Neoplasms - genetics; Polymerase Chain Reaction; Proteins - genetics; Sequence Homology, Nucleic Acid; Species Specificity; Trans-Activators; Transcription Factors; Genetic mapping; Human; Gene; A3-Chromosome
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1994.1493

The human β-catenin locus (CTNNB1) was mapped by in situ fluorescence analysis to band p21 on the short arm of chromosome 3, a region frequently affected by somatic alterations in a variety of tumors. PCR primers for the genomic amplification of β-catenin sequences were selected on the basis of homology to exon 4 of the Drosophila armadillo gene. Analysis of a panel of somatic cell hybrids confirmed the localization of β-catenin on human chromosome 3. Furthermore, exclusion mapping of three hybrids carrying defined fragments of the short arm of human chromosome 3 allowed us to determine the position of the CTNNB1 locus close to the marker D3S2 in 3p21.