UVB photoprotective capacity of hydrogels containing dihydromyricetin nanocapsules to UV-induced DNA damage

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 197; p. 111431
• Main Authors: Dalcin, Ana Júlia F., Roggia, Isabel, Felin, Sabrina, Vizzotto, Bruno S., Mitjans, Montserrat, Vinardell, Maria Pilar, Schuch, André P., Ourique, Aline F., Gomes, Patrícia
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021
• Subjects: Antioxidant; DNA damage; Eudragit; RS100®nanocosmetic; Solar radiation; Sun protection; Antioxidant; RS100®nanocosmetic; Solar radiation; Sun protection; Eudragit; DNA damage
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2020.111431

[Display omitted] •Hydrogels containing nanocapsules of the flavonoid DMY (NC-DMY) were produced.•NC-DMY demonstrated to be safe for topical application.•NC-DMY exhibited 50% SPF UVB and 99.9% protection against DNA lesion induction.•NC-DMY may be used as a promising photoprotective and antioxidant potential.•Being able to act eliminating free radicals formed by solar radiation. We evaluate the effect of cationic nanocapsules containing dihydromyricetin (DMY) flavonoid for safe topical use in photoprotection against UV-induced DNA damage. The stability was investigated for feasibility to produce hydrogels containing cationic nanocapsules of the flavonoid DMY (NC-DMY) for 90 days under three different storage conditions (4 ± 2 °C, 25 ± 2 °C, and 40 ± 2 °C), as well as evaluation of skin permeation and its cytotoxicity in skin cell lines. The physicochemical and rheological characteristics were maintained during the analysis period under the different aforementioned conditions. However, at 25 °C and 40 °C, the formulations indicated yellowish coloration and DMY content reduction. Therefore, the ideal storage condition of 4 °C was adopted. DMY remained in the stratum corneum and the uppermost layers of the skin. Regarding safety, all formulations demonstrated to be safe for topical application. NC-DMY exhibited a 50% Solar Protection Factor (SPF-DNA) against DNA damage caused by UVB radiation and demonstrated 99.9% protection against DNA lesion induction. These findings establish a promising formulation containing nanoencapsulated DMY flavonoids with a photoprotective and antioxidant potential of eliminating reactive oxygen species formed by solar radiation.