Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

• Published in: Acta cirurgica brasileira Vol. 23 Suppl 1; no. suppl 1; pp. 42 - 46
• Main Authors: Tucci Junior, Silvio, Carvalho, Roberto Marins de, Celini, Fábia Martins, Cologna, Adauto José, Suaid, Haylton Jorge, Tirapelli, Luis Fernando, Martins, Antonio Carlos Pereira
• Format: Journal Article
• Language: English
• Published: Brazil 2008
• Subjects: Animals; Biomarkers - blood; Chlorpromazine - pharmacology; Creatinine - blood; Disease Models, Animal; Dopamine Antagonists - pharmacology; Ischemia - complications; Jaundice, Obstructive - drug therapy; Kidney - blood supply; Kidney - drug effects; Kidney - physiopathology; Lipid Peroxidation - drug effects; Male; Malondialdehyde - blood; Nephrectomy; Rats; Rats, Wistar; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Time Factors; Urea - blood
• ISSN: 0102-8650; 1678-2674; 0102-8650
• DOI: 10.1590/S0102-86502008000700008

To evaluate the influence of chlorpromazine (CPZ) on renal function and lipid peroxidation in a rat model of kidney ischemia/reperfusion injury. Forty eight Wistar rats underwent a laparotomy for hilar clamping of left kidney with a bulldog clamp for 60 minutes followed by organ reperfusion and contralateral nephrectomy. Of these, 26 received 3mg/kg of CPZ intravenously 15 minutes before renal ischemia (G-E) while the remaining 22 were used as ischemic control group (G-C). Eleven rats of G-E and 8 of G-C were followed for blood urea nitrogen and creatinine determinations before renal ischemia and at 1st, 4th and 7th postoperative days. Samplings of left renal tissue were obtained at 5 minutes (5 rats from each group) and 24 hours (9 G-C and 10 of G-E) of reperfusion for malondialdehy (MDA) content determination. Controls of renal MDA content were determined in kidneys harvested from 6 additional normal rats. Acute renal failure occurred in all animals but levels of BUN and creatinine were significantly lower in G-E (p<0.001). MDA content rose strikingly at 5 minutes of reperfusion in both groups (p>0.05) and returned near to normal levels 24 hours later. CPZ conferred partial protection of renal function to kidneys submitted to ischemia/reperfusion injury that seems to be not dependent on inhibition of lipid peroxidation.