p53 Gene Is Up-Regulated during Skeletal Muscle Cell Differentiation

• Published in: Biochemical and biophysical research communications Vol. 192; no. 2; pp. 714 - 719
• Main Author: Halevy, O.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.04.1993; Elsevier
• Subjects: Animals; Biological and medical sciences; Cell Differentiation - genetics; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Cells, Cultured; Chickens; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genes, fos; Genes, p53; Molecular and cellular biology; Muscles - cytology; Muscles - metabolism; RNA, Messenger - metabolism; Up-Regulation; Rodentia; Cell differentiation; Gene expression; Striated muscle; Vertebrata; Mammalia; Mouse; Primary culture; Chicken; Aves; Satellite cell; Myogenic cell; Tumor suppressor gene
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1993.1473

Differentiation of cells in the mouse myogenic cell line C2, or a primary culture of chicken satellite cells was induced by low serum levels in the growth medium. Endogenous wild-type p53 mRNA was substantially expressed after approximately 5 h of incubation. Induction of p53 mRNA expression was also observed in cells treated with 10 −8 M retinoic acid, after 18 h of incubation. In both cases, the increase in p53 mRNA was transient. c-fos mRNA levels decreased rapidly and were barely detectable after 2 h of exposure to retinoic acid. The down-regulation of c-fos confirms its role in muscle cell differentiation, whereas the up-regulation of wild-type p53 suggests its role during this process.