A green decontamination technology through selective biomineralization of algicidal microorganisms for enhanced astaxanthin production from Haematococcus pluvialis at commercial scale

• Published in: Bioresource technology Vol. 332; p. 125121
• Main Authors: Yu, Byung Sun, Hong, Min Eui, Sung, Young Joon, Choi, Hong Il, Chang, Won Seok, Kwak, Ho Seok, Sim, Sang Jun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2021
• Subjects: Algicidal microorganism; Astaxanthin; Biomass; Biomineralization; CaCO3 biomineralization-based decontamination strategy; Chlorophyta; Decontamination; Haematococcus pluvialis; Mixotrophic culture mode; Xanthophylls; Mixotrophic culture mode; Algicidal microorganism; Astaxanthin; Haematococcus pluvialis; CaCO3 biomineralization-based decontamination strategy; CaCO biomineralization-based decontamination strategy
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2021.125121

[Display omitted] •Green decontamination tool was created to eliminate predators cidal to H. pluvialis.•CBDS was conducted at mild alkalinity and low CaCl2 for non-calcifying H. pluvialis.•CBDS showed an efficient removal of algicidal bacteria and fungi during cultivation.•Astaxanthin production using CBDS was significantly enhanced in H. pluvialis.•Large-scale outdoor astaxanthin production in H. pluvialis was improved by CBDS. Currently, there is a lack of an efficient, environmentally-benign and sustainable industrial decontamination strategy to steadily achieve improved astaxanthin production from Haematococcus pluvialis under large-scale outdoor conditions. Here, this study demonstrates for the first time that a CaCO3 biomineralization-based decontamination strategy (CBDS) is highly efficient in selectively eliminating algicidal microorganisms, such as bacteria and fungi, during large-scale H. pluvialis cultivation under autotrophic and mixotrophic conditions, thereby augmenting the astaxanthin productivity. Under outdoor AT and MT conditions, the average astaxanthin productivity of H. pluvialis using CBDS in a closed photobioreactor system was substantially increased by 14.85- (1.19 mg L−1 d−1) and 13.65-fold (2.43 mg L−1 d−1), respectively, compared to the contaminated H. pluvialis cultures. Given the exponentially increasing demand of astaxanthin, a natural anti-viral, anti-inflammatory, and antioxidant drug, CBDS will be a technology of interest in H. pluvialis-based commercial astaxanthin production which has been hindered by the serious biological contaminations.