Tunable multiplexed fluorescence biosensing platform for simultaneous and selective detection of paraquat and carbendazim pesticides

•BPNSs as a single acceptor paired with tunable multicolor UCNPs as donors.•A novel multiplexed FRET sensor was developed for paraquat and carbendazim detection.•The detection limits of paraquat and carbendazim were 0.18 ng/mL and 0.45 ng/mL.•This work offers a universal biosensing platform in advan...

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Bibliographic Details
Published inFood chemistry Vol. 388; p. 132950
Main Authors Wang, Li, Haruna, Suleiman A., Ahmad, Waqas, Wu, Jizhong, Chen, Quansheng, Ouyang, Qin
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 15.09.2022
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Summary:•BPNSs as a single acceptor paired with tunable multicolor UCNPs as donors.•A novel multiplexed FRET sensor was developed for paraquat and carbendazim detection.•The detection limits of paraquat and carbendazim were 0.18 ng/mL and 0.45 ng/mL.•This work offers a universal biosensing platform in advancing multiplexed analysis. The monitoring of multiple pesticides commonly used in food is a prerequisite for public health safety. Herein, a multiplexed biosensor based on fluorescence resonance energy transfer (FRET) from multicolor upconversion nanoparticles (UCNPs)to single black phosphorus nanosheets (BPNSs) was successfully developed for simultaneous and selective detection of paraquat and carbendazim pesticides. Due to the strong π-π stacking interactions, aptamers functionalized UCNPs may adsorb on the BPNSs surface, allowing strong upconversion fluorescence quenching. In the presence of paraquat and carbendazim, the aptamers preferentially integrated with their corresponding targets and altered the aptamer’s conformation, restoring the fluorescence. An excellent linear correlation was observed from 1.0 to 1.0 × 105 ng/mL, with a limit of detection of 0.18 ng/mL for paraquat and 0.45 ng/mL for carbendazim. The developed aptasensor was further validated by commercial enzyme-linked immunoassays without significant differences in practical detection. Additionally, this work offers new insights into monitoring multiple targets simultaneously.
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ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2022.132950